| 货号 | 4716S |
| 反应种属 | Human/Mouse/Rat/D.melanogaster/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | RACK1 Antibody recognizes endogenous levels of total RACK1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
| 参考文献 | 1 . Ron, D. et al. (1994) Proc Natl Acad Sci U S A 91, 839-43. 2 . López-Bergami, P. et al. (2005) Mol Cell 19, 309-20. 3 . McCahill, A. et al. (2002) Mol Pharmacol 62, 1261-73. 4 . Nilsson, J. et al. (2004) EMBO Rep 5, 1137-41. 5 . Chang, B.Y. et al. (2002) Oncogene 21, 7619-29. |
Western blot analysis of extracts from various cell lines, using RACK1 Antibody. 使用RACK1 Antibody，免疫印迹（Western Blot）分析不同细胞中RACK1蛋白水平。 |
