| 货号 | 3930S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Rab3A Antibody detects endogenous levels of total Rab3A protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Rab family of proteins includes small, monomeric GTPases essential for regulating intracellular vesicle trafficking. Members of the Rab3 subfamily, including Rab3A-3D, are involved in the exocytosis of neurotransmitters and hormones (1). Rab3A is primarily expressed in neurons (2), neuroendocrine cells (such as rat PC-12 cells), and in human pancreatic β cells (3, 4). By acting as a molecular switch between active, GTP-bound Rab3A and the inactive, GDP-bound form, Rab3a inhibits synaptic vesicle and chromaffin granule secretion during late membrane release (5,6). Loss-of-function studies suggest Rab3A is involved in controlling synaptic vesicle targeting and docking at the active zone (7). Through binding to its direct effector Rabphillin, Rab3A also orchestrates the coupling between synaptic vesicle exocytosis and endocytosis (8). |
| 存放说明 | -20C |
| 计算分子量 | 25 |
| 参考文献 | 1 . Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17. 2 . Geppert, M. et al. (1994) Nature 369, 493-7. 3 . Weber, E. et al. (1996) J Biol Chem 271, 6963-71. 4 . Yaekura, K. et al. (2003) J Biol Chem 278, 9715-21. 5 . Johannes, L. et al. (1994) EMBO J 13, 2029-37. 6 . Geppert, M. et al. (1997) Nature 387, 810-4. 7 . Leenders, A.G. et al. (2001) Mol Biol Cell 12, 3095-102. 8 . Coppola, T. et al. (2001) J Cell Sci 114, 1757-64. |
Western blot analysis of extracts from human brain, mouse brain, rat brain and PC-12 cells using Rab3A Antibody. 对人脑、小鼠脑、大鼠脑和PC-12细胞抽提液使用Rab3A Antibody进行Western blot分析。 |
