| 货号 | 4314S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Rab25 Antibody detects endogenous levels of total Rab25 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rab11a, Rab11b and Rab25 are members of the Rab11 subfamily of small Ras-like GTPases. Unlike universally expressed Rab11, typical Rab25 expression appears to be limited to gastrointestinal mucosa, kidney, and lung (1). Rab25 can associate with apical recycling vesicles to help regulate apical vesicle trafficking (2,3). Atypical Rab25 expression has been associated with various forms of cancer. Increased Rab25 expression is associated with aggressive growth in ovarian and breast cancer, where Rab25 may inhibit apoptosis and promote cancer cell proliferation and invasion through regulation of vesicle transport and cellular motility (4-7). Interaction between Rab25 and β1 integrin promotes vesicle-mediated transport of integrin to pseudopodial tip membranes, fostering the persistent invasion of tumor cells (8). Conversely, the reported loss of Rab25 expression in a number of breast cancer cases has an unclear effect on cancer pathogenesis (9). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
| 参考文献 | 1 . Goldenring, J.R. et al. (1993) J Biol Chem 268, 18419-22. 2 . Casanova, J.E. et al. (1999) Mol Biol Cell 10, 47-61. 3 . Wang, X. et al. (2000) J Biol Chem 275, 29138-46. 4 . Cheng, K.W. et al. (2004) Nat Med 10, 1251-6. 5 . Cheng, K.W. et al. (2005) Cancer Res 65, 2516-9. 6 . Chia, W.J. and Tang, B.L. (2009) Biochim Biophys Acta 1795, 110-6. 7 . Tang, B.L. and Ng, E.L. (2009) Cell Motil Cytoskeleton 66, 365-70. 8 . Caswell, P.T. et al. (2007) Dev Cell 13, 496-510. 9 . Cheng, J.M. et al. (2010) Int J Cancer 126, 2799-812. |
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected (+) with human Rab25, using Rab25 Antibody. 对COS-7细胞，未转染或转染人Rab25，使用Rab25 Antibody进行Western blot分析。 | |
Confocal immunofluorescent analysis of MCF-7 cells (upper) and HeLa cells (lower), using Rab25 Antibody (green). Actin filaments were labeled using DY-554 Phallodin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对MCF-7细胞（上）和HeLa细胞（下）使用Rab25 Antibody（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 |
