| 货号 | 4157S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PZR Antibody detects endogenous levels of total PZR protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8). |
| 存放说明 | -20C |
| 计算分子量 | 30-50 |
| 参考文献 | 1 . Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72. 2 . Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9. 3 . Kusano, K. et al. Endothelium 15, 127-36. 4 . Zhao, R. et al. (2002) J Biol Chem 277, 7882-8. 5 . Zhao, R. et al. (2003) J Biol Chem 278, 42893-8. 6 . Zannettino, A.C. et al. (2003) Biochem J 370, 537-49. 7 . Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33. 8 . Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38. |
Western blot analysis of extracts from HeLa and HEK293T cells using PZR Antibody.使用PZR抗体对HeLa和HEK293T细胞提取物进行western blot分析。 |
