| 货号 | 2784S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Pyruvate Dehydrogenase Antibody detects endogenous levels of total α1 and α2 subunits of pyruvate dehydrogenase protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: α and β. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
| 参考文献 | 1 . Strumiło, S. (2005) Acta Biochim Pol 52, 759-64. 2 . Stacpoole, P.W. et al. (2003) Curr Gene Ther 3, 239-45. |
Western blot analysis of extracts from various cell types using Pyruvate Dehydrogenase Antibody. 对多个细胞系使用Pyruvate Dehydrogenase Antibody进行Western blot分析。 |
