| 货号 | 2449S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | PRMT1 (A33) Antibody detects endogenous levels of total PRMT1 protein (all three isoforms). The antibody does not cross-react with other PRMT proteins. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein arginine N-methyltransferase 1 (PRMT1) is a member of the protein arginine N-methyltransferase (PRMT) family of proteins that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (1). Though all PRMT proteins catalyze the formation of mono-methyl arginine, Type I PRMTs (PRMT1, 3, 4, and 6) add an additional methyl group to produce an asymmetric di-methyl arginine while Type II PRMTs (PRMT 5 and 7) produce symmetric di-methyl arginine (1). Mono-methyl arginine, but not di-methyl arginine, can be converted to citrulline through deimination catalyzed by enzymes such as PADI4 (2). Most PRMTs, including PRMT1, methylate arginine residues found within glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (1). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within PGM (proline-, glycine-, methionine-rich) motifs (3). PRMT1 methylates Arg3 of histone H4 and cooperates synergistically with p300/CBP to enhance transcriptional activation by nuclear receptor proteins (4-6). In addition, PRMT1 methylates many non-histone proteins, including the orphan nuclear receptor HNF4 (6), components of the heterogeneous nuclear ribonucleoprotein (hnRNP) particle (7), the RNA binding protein Sam68 (8), interleukin enhancer-binding factor 3 (ILF3) (9) and interferon-α and β receptors (10). These interactions suggest additional functions in transcriptional regulation, mRNA processing and signal transduction. Alternative mRNA splicing produces three enzymatically active PMRT1 isoforms that differ in their amino-terminal regions (11). PRMT1 is localized to the nucleus or cytoplasm, depending on cell type (12,13) and appears in many distinct protein complexes. ILF3, TIS21 and the leukemia-associated BTG1 proteins bind PRMT1 to regulate its methyltransferase activity (9,14). |
| 存放说明 | -20C |
| 计算分子量 | 41 |
| 参考文献 | 1 . Bedford, M.T. and Richard, S. (2005) Mol Cell 18, 263-72. 2 . Wang, Y. et al. (2004) Science 306, 279-83. 3 . Cheng, D. et al. (2007) Mol Cell 25, 71-83. 4 . Wang, H. et al. (2001) Science 293, 853-857. 5 . Strahl, B.D. et al. (2001) Curr. Biol. 11, 996-1000. 6 . Barrero, M.J. and Malik, S. (2006) Mol. Cell 24, 233-243. 7 . Nichols, R.C. et al. (2000) Exp. Cell Res. 256, 522-532. 8 . Côté, J. et al. (2003) Mol. Biol. Cell 14, 274-287. 9 . Tang, J. et al. (2000) J. Biol. Chem. 275, 19866-19876. 10 . Abramovich, C. et al. (1997) EMBO J. 16, 260-266. 11 . Scorilas, A. et al. (2000) Biochem. Biophys. Res. Commun. 278, 349-359. 12 . Frankel, A. et al. (2002) J. Biol. Chem. 277, 3537-3543. 13 . Herrmann, F. et al. (2005) J. Biol. Chem. 280, 38005-38010. 14 . Lin, W.J. et al. (1996) J. Biol. Chem. 271, 15034-15044. |
Confocal immunofluorescent analysis of either untreated HeLa (left), HCT-116 (middle) or SK-OV-3 (right) cells using PRMT1 (A33) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用PRMT1 (A33) Antibody(绿色)标记，共聚焦免疫荧光分析未处理的HeLa细胞(left)、HCT-116 (中图)或SK-OV-3 (右图)。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of cell lysates from A549, NIH/3T3, H-4-II-E and COS cells using PRMT1 Antibody. 使用PRMT1 Antibody，免疫印迹(Western blot)分析A549、NIH/3T3、H-4-II-E和COS细胞中的PRMT1蛋白水平。 |
