| 货号 | 3803S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PPIG Antibody detects endogenous levels of total PPIG protein. This antibody also recognizes a unidentified protein at 49 kDa. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PPIG belongs to a highly conserved class of cyclophilins that function as peptidyl-prolyl-isomerases (PPIases) to catalyze the conversion of cis-proline to trans-proline in a polypeptide chain (1-4). PPIG contains an amino-terminal cyclophilin domain followed by Nopp140 repeats that are involved in its function as a nuclear chaperone (5). The carboxy-terminal of PPIG contains a SR (arginine-serine dipeptide repeat) domain (3,4) that is involved in pre-mRNA splicing and processing (6). PPIG interacts with the carboxy-terminal domain of RNA polymerase II as well as several other SR family splicing factors. These interactions lead to changes in localization and conformation and suggest a regulatory role in transcription and pre-mRNA splicing in the elongating RNA polymerase complex (7,8). PPIG is found in the nuclear matrix and nuclear speckles and is involved in the regulation of gene expression. PPIG shows a predominantly diffuse cytoplasmic distribution at the onset of mitosis, and in late telophase the isomerase is recruited to the newly formed nuclei (9). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Fischer, G. et al. (1989) Nature 337, 476-8. 2 . Freskgård, P.O. et al. (1992) Science 258, 466-8. 3 . Nestel, F.P. et al. (1996) Gene 180, 151-5. 4 . Mortillaro, M.J. and Berezney, R. (1998) J Biol Chem 273, 8183-92. 5 . Meier, U.T. and Blobel, G. (1992) Cell 70, 127-38. 6 . Zahler, A.M. et al. (1993) Science 260, 219-22. 7 . Lin, C.L. et al. (2004) Biochem Biophys Res Commun 321, 638-47. 8 . Bourquin, J.P. et al. (1997) Nucleic Acids Res 25, 2055-61. 9 . Dubourg, B. et al. (2004) J Biol Chem 279, 22322-30. |
Western blot analysis of extracts from various cell lines using PPIG Antibody. 使用PPIG Antibody，免疫印迹（Western Blot）分析在不同细胞系中PPIG蛋白水平。 |
