| 货号 | 2430S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PPARγ (D69) Antibody detects endogenous levels of total PPARγ protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).过氧化物酶体增殖物激活受体γ（PPARγ）是配体激活的核受体超家族成员，作为转录激活剂起作用（1）。 PPARγ优先在脂肪细胞、血管平滑肌细胞和巨噬细胞表达（2）。除此之外，它在介导脂肪细胞分化和脂质代谢中起作用（2），PPARγ调节胰岛素敏感性、细胞增殖和炎症（3）。 PPARγ的转录活性可以被MAP激酶通过磷酸化PPARγ丝氨酸（84位）抑制（4,5）。 |
| 存放说明 | -20C |
| 计算分子量 | 53, 57 |
| 参考文献 | 1 . Tontonoz, P. et al. (1995) Curr Opin Genet Dev 5, 571-6. 2 . Rosen, E.D. et al. (1999) Mol Cell 4, 611-7. 3 . Murphy, G.J. and Holder, J.C. (2000) Trends Pharmacol Sci 21, 469-74. 4 . Camp, H.S. and Tafuri, S.R. (1997) J. Biol. Chem. 272, 10811-10816. 5 . Adams, M. et al. (1997) J. Biol. Chem. 272, 5128-5132. |
Western blot analysis of extracts from NIH/3T3 and NIH/3T3-L1 cells (differentiated 6 days) using PPARγ (D69) Antibody.Western blot方法检测NIH/3T3细胞和NIH/3T3-L1细胞（分化6天）提取物，使用的抗体为PPARγ (D69) Antibody. |
