| 货号 | 2582S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PP1α Antibody detects endogenous levels of total PP1α protein. This antibody does not cross-react with PP1δ or PP1γ. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).1型蛋白磷酸酶(PP1)是真核细胞中一种高度保守的丝氨酸/苏氨酸磷酸酶。PP1具有四个异构体：PP1α, PP1δ, PP1γ1 和PP1γ2 (1)。PP1的一个生物学功能是参与细胞周期调节(1)。有描述认为PP1在有丝分裂期能够去磷酸化Rb 和cdc25(2,3)。Cdc20介导的PP1α第320位苏氨酸细胞周期依赖性磷酸化能够抑制PP1α的活性(4)。 |
| 存放说明 | -20C |
| 计算分子量 | 38 |
| 参考文献 | 1 . Rubin, E. et al. (1998) Front Biosci 3, D1209-19. 2 . Durfee, T. et al. (1993) Genes Dev 7, 555-69. 3 . Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39. 4 . Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73. |
Western blot analysis of purified recombinant PP1α protein (lane 1), and extracts from C6, NIH/3T3 and HeLa cells (lanes 2-4), using PP1α Antibody.Western blot 方法检测纯化的重组PP1α 蛋白（泳道1）和C6, NIH/3T3 和HeLa 细胞提取物（泳道2-4），使用的抗体为 PP1α Antibody。 |
