| 货号 | 14171S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PNUTS Antibody recognizes endogenous levels of total PNUTS protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein phosphatase-1 nuclear targeting subunit (PNUTS) is one of the key regulators of protein phosphatase 1 (PP1) in the nucleus (1). Via interaction with PP1, PNUTS plays an essential role in multiple cellular processes, including chromatin decondensation (2), DNA damage response (3), and cardiomyocyte apoptosis (4). Notably, PNUTS also regulates the activity of two key tumor suppressors, Rb and p53, through inhibition of PP1 mediated dephosphorylation (5-7). Research studies indicate that PNUTS also sequesters PTEN in the nucleus through direct interaction and inhibits its tumor suppressor function (8). PNUTS is ubiquitously expressed and elevated PNUTS expression is observed in various cancers such as esophageal carcinoma, squamous cell carcinoma, and prostate cancer (1,8). |
| 存放说明 | -20C |
| 计算分子量 | 115 |
| 参考文献 | 1 . Allen, P.B. et al. (1998) J Biol Chem 273, 4089-95. 2 . Landsverk, H.B. et al. (2005) Biochem J 390, 709-17. 3 . Landsverk, H.B. et al. (2010) EMBO Rep 11, 868-75. 4 . Boon, R.A. et al. (2013) Nature 495, 107-10. 5 . Lee, S.J. et al. (2007) Cell Death Differ 14, 1106-16. 6 . De Leon, G. et al. (2008) Cancer Biol Ther 7, 833-41. 7 . Choy, M.S. et al. (2014) Proc Natl Acad Sci U S A 111, 4097-102. 8 . Kavela S et al. (2013) Cancer Res 73, 205–14 |
Western blot analysis of extracts from MCF7, HT-29 and HeLa cells using PNUTS Antibody. | |
Immunoprecipitation of PNUTS from MCF7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or PNUTS Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PNUTS Antibody. |
