| 货号 | 2863S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Plectin-1 Antibody detects endogenous levels of total plectin-1 protein. The antibody is expected to cross-react with all isoforms of plectin-1 based on the sequence of the immunogenic peptide. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Plectin is a large, widely expressed protein that crosslinks the intermediate filament and actin cytoskeleton, mechanically stabilizing cells and tissues. Plectin also plays a role in the regulation of actin dynamics and acts as a scaffold for signaling molecules (1). Plectin is important in the stabilization of hemidesmosomes, crosslinking them to the intermediate filament network. Mutations in plectin and other genes coding for hemidesmosomal proteins can cause epidermolysis bullosa, a condition manifested by fragile skin and frequent blistering (1,2). Plectin has been shown too be involved in several signaling cascades. It signals to PKC through binding to and sequestering of RACK1, the receptor for activated C kinase 1 (3,4). Plectin is also involved in the regulation of cytokeratin architecture and cell stress response (4), signaling through the chemokine receptor CXCR4 (5) and regulation of AMP-activated protein kinase (AMPK) activity and signaling in mouse myotubes (6). |
| 存放说明 | -20C |
| 计算分子量 | 400-500 |
| 参考文献 | 1 . Wiche, G. (1998) J Cell Sci 111 ( Pt 17), 2477-86. 2 . Pfendner, E. et al. (2005) Exp Dermatol 14, 241-9. 3 . Osmanagic-Myers, S. and Wiche, G. (2004) J Biol Chem 279, 18701-10. 4 . Osmanagic-Myers, S. et al. (2006) J Cell Biol 174, 557-68. 5 . Ding, Y. et al. (2008) Exp Cell Res 314, 590-602. 6 . Gregor, M. et al. (2006) J Cell Sci 119, 1864-75. |
Western blot analysis of extracts from various cell types using Plectin-1 Antibody. Molecular weights were determined using a pre-stained molecular weight marker (not shown). 使用Plectin-1 Antibody，免疫印迹（Western Blot）分析不同细胞中Plectin-1蛋白水平。使用预染标记物确定分子量大小（没有显示）。 |
