| 货号 | 3832S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PLD1 Antibody detects endogenous levels of total PLD1 protein. This antibody does not cross-react with PLD2. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified so far. Both are regulated by protein kinases, small GTPases and Ca2+ (1). PLD1 is phosphorylated at Ser2, Ser561 and Thr147 by PKC (2,3). Phosphorylation at Thr147 and Ser561 regulates PLD1 activity (3). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
| 参考文献 | 1 . Exton, J.H. (1999) Biochim Biophys Acta 1439, 121-33. 2 . Kim, Y. et al. (1999) Biochemistry 38, 10344-51. 3 . Kim, Y. et al. (2000) J. Biol. Chem. 275, 13621-13627. |
Western blot analysis of extracts from HeLa cells stimulated with PMA for 0, 10, 20 and 30 minutes using PLD1 Antibody. 对PMA处理0，10，20和30分钟的HeLa细胞抽提物，使用PLD1抗体进行Western blot分析。 |
