| 货号 | 3872S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PLCgamma2 Antibody detects endogenous levels of total PLCγ2 protein. This antibody does not cross-react with PLCγ1. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. 2 . Smrcka, A.V. et al. (1991) Science 251, 804-7. 3 . Taylor, S.J. et al. (1991) Nature 350, 516-8. 4 . Yue, C. et al. (1998) J Biol Chem 273, 18023-7. 5 . Yue, C. et al. (2000) J Biol Chem 275, 30220-5. 6 . Margolis, B. et al. (1989) Cell 57, 1101-7. 7 . Kim, H.K. et al. (1991) Cell 65, 435-41. 8 . Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7. 9 . Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601. 10 . Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9. |
Western blot analysis of extracts from NIH/3T3 (lanes 1, 3, 5) and Ramos (lanes 2, 4, 6) cells, using PLCγ1 Antibody #2822 (lanes 1 and 2), PLCgamma2 Antibody (lanes 5 and 6) or both antibodies (lanes 3 and 4). Results show that PLCγ2 Antibody is specific to the 150 kDa PLCγ2 band detected in Ramos cells, while PLCγ1 Antibody #2822 is specifc to the 160 kDa PLCγ1 band detected in both Ramos and NIH/3T3 cells. 对NIH/3T3（列1，3，5）和Ramos细胞（列2，4，6）抽提液使用PLCγ1抗体#2822（列1，2）、PLCγ2抗体（列5，6）或两种抗体均用（列3，4）进行Western blot分析。结果显示PLCγ2抗体特异性的对Ramos细胞150 kDa PLCγ2条带，PLCγ1 抗体#2822特异性的对Ramos和NIH/3T3细胞160 kDa PLCγ1条带。 | |
Western blot analysis of extracts from Ramos (lane 1), THP1 (lane 2), U-937 (lane 3) cells and mouse spleenocytes (lane 4), using PLCγ2 Antibody. 对Ramos（列1）、THP1（列2）和U-937（列3）细胞和小鼠脾脏（列4）使用PLCγ2抗体进行Western blot分析。 |
