| 货号 | 3072S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PKR Antibody detects endogenous levels of PKR. This antibody does not cross-react with other related proteins. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the alpha subunit of the initiation factor eIF2 (eIF2alpha) and also controls the activation of several transcription factors such as NF-kappaB, p53 and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation (1,2). Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation at threonine 446 and threonine 451 in the activation loop. Substitution of threonine 451 to alanine completely inactivates PKR, while a mutant with a threonine 446 to alanine substitution was partially active (3). Recently, activation of PKR has been implicated in the pathologies of neurodegenerative diseases, including Alzheimer disease (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 74 |
| 参考文献 | 1 . Williams, B.R. (1999) Oncogene 18, 6112-6120. 2 . Gil, J. and Esteban, M. (2000) Apoptosis 5, 107-114. 3 . Romano, P. R. et al. (1998) Mol. Cell. Biol. 18, 2282-2297. 4 . Peel, A.L. and Bredesen, D.E. (2003) Neurobiol Dis 14, 52-62. 5 . Peel, A.L. (2004) J Neuropathol Exp Neurol 63, 97-105. |
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha/beta-treated (1000 U/ml for 18 hours), and subsequently untreated or calyculin A-treated ( 0.1 µM for 15 minutes), using PKR Antibody. 对HeLa细胞，不处理或加入IFNα/IFNβ 1000U/ml处理18小时，再用calyculin A（0.1 uM）处理15分钟，使用PKR Antibody进行Western blot分析。 |
