| 货号 | 3186S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PKM1/2 Antibody detects endogenous levels of total PKM (including M1 and M2) protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4). A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Christofk, H.R. et al. (2008) Nature 452, 230-3. 2 . Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8. 3 . Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29. 4 . Israelsen, W.J. et al. (2013) Cell 155, 397-409. |
Western blot analysis of extracts from various cell lines using PKM1/2 Antibody. 对多个细胞系使用PKM1/2抗体进行Western blot分析。 | |
Western blot analysis of extract from mouse muscle using PKM1/2 Antibody. 对小鼠肌肉抽提液使用PKM1/2抗体进行Western blot分析。 |
