| 货号 | 3927S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | PKA RI-α/β Antibody detects endogenous levels of total PKA RI-α and PKA RI-β protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (2). Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9) (3-5). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259) (6). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197 (7). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Montminy, M. (1997) Annu Rev Biochem 66, 807-22. 2 . DellAcqua, M.L. and Scott, J.D. (1997) J Biol Chem 272, 12881-4. 3 . Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869. 4 . Gonzalez, G.A. and Montminy, M.R. (1989) Cell 59, 675-680. 5 . Fang, X. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 11960-11965. 6 . Dumaz N and Marais R (2003) J Biol Chem 278, 29819–23 7 . Moore, M.J. et al. (2002) J. Biol. Chem. 277, 47878-47884. |
Western blot analysis of extracts from U118MG and CAD cells using PKA RI-α/β Antibody. 对U118MG和CAD细胞抽提液使用PKA RI-α/β抗体进行Western blot分析。 | |
Confocal immunofluorescent analysis of U-118 MG cells using PKA RI-α/β Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
