| 货号 | 3296S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PIP5K1C Antibody detects endogenous levels of total PIP5K1C protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphatidylinositol-4-phosphate 5-kinases (PIP5K) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that also regulates some proteins and cellular processes directly. There are two subfamilies of PIP5Ks, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP5K1s use PtdIns4P as a substrate, whereas PIP5K2s use PtdIns5P (1,2). In mammalian cells, three isoforms of each PIP5K1 and PIP5K2 subfamily, encoded by distinct genes, have been characterized and named α, β and γ (3-7), and additional PIPKs likely remain to be discovered. All PIP5K isoforms are stimulated by phosphatidic acid, are extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 87, 90 |
| 参考文献 | 1 . Rameh, L.E. et al. (1997) Nature 390, 192-6. 2 . Zhang, X. et al. (1997) J Biol Chem 272, 17756-61. 3 . Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4. 4 . Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43. 5 . Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8. 6 . Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299 7 . Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60. 8 . Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99. |
Western blot analysis of extracts from various cell lines using PIP5K1C Antibody. 对多个细胞系使用PIP5K1C抗体进行Western blot分析。 |
