| 货号 | 3722S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Pin1 Antibody detects endogenous levels of total Pin1 protein. The antibody does not cross-react with other proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Pin1, a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2/M transition of the mammalian cell cycle (1-6). Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).Pin1是小细胞蛋白肽脯氨酰异构酶（PPIase）家庭成员，参与哺乳动物细胞周期的G2/M期转换(1-6)。Pin1是一个18 kDa的小蛋白，含有两个独立的功能结构域：一个氨基末端WW结构域和一个羧基末端Pplase结构域。Pin1能够与几个有丝分裂磷蛋白相互作用，包括Plk1、cdc25C和cdc27，而且被认为能够充当这些靶分子的磷酸化依赖性Pplase(7-9)。 |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . Lu, P.J. et al. (1999) Science 283, 1325-8. 2 . Verdecia, M. A. et al. (2000) Nat. Struct. Biol. 7, 639-643. 3 . Lu, K. P. et al. (1996) Nature 380, 544-547. 4 . Zhou, X. Z. et al. (2000) Mol. Cell 6, 873-883. 5 . Wu, X. et al. (2000) EMBO J. 19, 3727-3738. 6 . Winkler, K. E. et al. (2000) Science 287, 1644-1647. 7 . Crenshaw, D. G. et al. (1998) EMBO J. 17, 1315-1327. 8 . Shen, M. et al. (1998) Genes Dev. 12, 706-720. 9 . Yaffe, M.B. et al. (1997) Science 278, 1957-60. |
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using Pin1 Antibody.Western blot 方法检测HeLa, NIH/3T3, PC12和COS cells,使用的抗体为Pin1 Antibody。 |
