| 货号 | 2907S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Pim-1 Antibody recognizes endogenous levels of total Pim-1 protein. This antibody does not cross-react with other Pim proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 34, 44 |
| 参考文献 | 1 . Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15. 2 . Selten, G. et al. (1986) Cell 46, 603-11. 3 . Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12. 4 . Dautry, F. et al. (1988) J Biol Chem 263, 17615-20. 5 . Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8. 6 . Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55. 7 . Leverson, J.D. et al. (1998) Mol Cell 2, 417-25. 8 . Winn, L.M. et al. (2003) Cell Cycle 2, 258-62. 9 . Pasqualucci, L. et al. (2001) Nature 412, 341-6. 10 . Kim, O. et al. (2004) Oncogene 23, 1838-44. 11 . Aho, T.L. et al. (2004) FEBS Lett 571, 43-9. 12 . Yan, B. et al. (2003) J Biol Chem 278, 45358-67. 13 . Saris, C.J. et al. (1991) EMBO J 10, 655-64. |
Western blot analysis of extracts from K562, Raji and NK-92 cell lines using Pim-1 Antibody.Western免疫印迹。用Pim-1 Antibody研究K562, Raji 和 NK-92 细胞系的细胞提取液。 | |
Western blot analysis of recombinant Pim-1, -2 and -3 (5 ng) using Pim-1 Antibody.Western免疫印迹。用Pim-1 Antibody研究重组的Pim-1, -2 和 -3(5 ng) 蛋白。 |
