| 货号 | 4255S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PI3 Kinase p110α Antibody detects endogenous levels of total PI3K p110α protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Cantley, L.C. (2002) Science 296, 1655-7. 2 . Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. 3 . Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. 4 . Stoyanov, B. et al. (1995) Science 269, 690-3. |
Western Blot analysis of Jurkat cell extracts treated with LY294002 (PI3 Kinase Inhibitor) #9901 or Calyculin A #9902 using PI3 Kinase p110α Antibody. Western blot分析LY294002 (PI3 Kinase Inhibitor) #9901 或者 Calyculin A #9902. K562 处理的Jurkat细胞提取物，所用抗体为 PI3 Kinase p110α Antibody。 |
