| 货号 | 2446S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | PDI Antibody detects endogenous levels of total PDI protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3). |
| 存放说明 | -20C |
| 计算分子量 | 57 |
Western blot analysis of extracts from various cell lines using PDI Antibody. 对多个细胞系使用PDI Antibody进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PDI Antibody. 对石蜡包埋的人乳腺癌使用PDI Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI Antibody. 对石蜡包埋的人肺癌使用PDI Antibody进行免疫组化分析。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI Antibody (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对NIH/3T3细胞使用PDI Antibody（绿色）和β-Actin (8H10D10) Mouse mAb #3700（红色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 |
