| 货号 | 3585S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PDE5 (P434) Antibody detects endogenous levels of total PDE5 protein. The antibody cross-reacts with a 35-40 kDa protein of unknown origin. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The superfamily of phosphodiesterases (PDEs) catalyses the hydrolysis of 3,5-cyclic nucleotides into the corresponding nucleotide 5-monophosphates. PDE5 is a cGMP-specific enzyme that contains two cGMP binding sites in its amino-terminal regulatory domain (1). Elevation of cGMP levels causes activation of PKG, which phosphorylates PDE5 at Ser102 (2). Phosphorylation of PDE5 stimulates its enzymatic activity and enhances cGMP binding affinity in the regulatory domain, leading to a decrease in intracellular cGMP levels (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Western blot analysis of extracts from LN-18 cells using PDE5 (P434) Antibody. 对LN-18细胞抽提液使用PDE5 (P434)抗体进行Western blot分析。 | |
Immunoprecipitation of PDE5 from LN-18 cells using PDE5 (P434) Antibody followed by western blot using the same antibody. Lane 1 is 5% input. 使用PDE5 (P434)抗体对LN-18细胞裂解液进行PDE5的免疫沉淀，之后Western blot分析。列1是5%的输入对照。 |
