货号 | 6924S |
反应种属 | Human/Mouse/Rat/Monkey |
来源宿主 | Rabbit |
应用 | W/IP/IF-IC |
目标/特异性 | PCK2 Antibody recognizes endogenous levels of total PCK2 protein. |
使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) |
供应商 | CST |
背景 | PCK2 (PEPCK2 or PEPCK-M) encodes an isoform of phosphoenolpyruvate carboxykinase (PEPCK) that is found in the mitochondria of renal and hepatic tissues (1). PEPCK is involved in gluconeogenesis, the process of generating glucose from non-carbohydrate substrates such as lactate and glycerol (2). PEPCK catalyzes the reaction whereby oxaloacetate becomes phosphoenolpyruvate, a rate limiting step in the metabolic process (2). The predominant function of PCK2 is to process the lactate that is continually produced by red blood cells in the liver and kidney (1). |
存放说明 | -20C |
计算分子量 | 71 |
Western blot analysis of extracts from various cell lines using PCK2 Antibody. Wetern blot检测多种细胞系提取物,所用抗体为PCK2 Antibody。 | |
Confocal immunofluorescent analysis of C2C12 cells using PCK2 Antibody (green) showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测C2C12细胞,显示蛋白与线粒体(红)的共定位,所用抗体为PCK2 Antibody (绿),蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) |