| 货号 | 2130S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | P-Cadherin Antibody recognizes endogenous levels of total P-cadherin protein. The antibody does not cross-react with other cadherin family members. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot anlaysis of extracts from A431, Panc1 and HUVE cells, using P-Cadherin Antibody.使用P-Cadherin 抗体对A431，Panc1和HUVE细胞提取物进行western blot分析。 | |
Immunofluorescent analysis of A431 cells, using P-Cadherin Antibody (left). Nuclei in the same cells were stained with DAPI (right).使用P-Cadherin 抗体（左）对A431细胞进行免疫组化分析。同个细胞中的细胞核使用DAPI染色（右）。 | |
Confocal immunofluorescent images of A431 cells labeled with P-Cadherin Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).使用P-Cadherin 抗体（绿色，左）对异型对照（右）对A431细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用Alexa Fluor 555鬼笔环肽（红色）进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ™（DNA荧光染料）。 |
