| 货号 | 4942S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-Binding domain, which is thought to interact with the tumor suppressor protein hDlg (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclinB has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (1). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4).PBK/TOPK是一种丝氨酸/苏氨酸激酶，在有丝分裂过程中被磷酸化和活化(1)。PBK/TOPK由激酶亚结构域和一个羧基末端PDZ-结合结构域，该结构域被认为能够和肿瘤抑制蛋白hDlg相互作用(1)。在高度增殖的恶性肿瘤细胞系中已观察到PBK/TOPK的表达增加，而在终端分化的HL-60白血病细胞中观察到PBK/TOPK的显著下调(2,3)。PMA-诱导的针对PBK/TOPK的激酶活性也被观察到，且cdc2/cyclinB被认为可以离体磷酸化PBK/TOPK，可能的位点是第9位苏氨酸(1)。PBK/TOPK的潜在底物包括p38 MAPK 和c-Myc (3,4)。 |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Western blot analysis of extracts from NIH/3T3 and PC12 cells, using PBK/TOPK Antibody.Western blot 方法检测NIH/3T3细胞和PC12细胞的提取物，使用的抗体为PBK/TOPK Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing membrane and cytoplasmic localization, using PBK/TOPK Antibody.免疫组织化学方法检测石蜡包埋人类结肠癌组织，显示膜定位和胞浆定位，使用的抗体为PBK/TOPK Antibody. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using PBK/TOPK Antibody.免疫组织化学方法检测石蜡包埋人类肺癌组织，使用的抗体为PBK/TOPK Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PBK/TOPK Antibody.免疫组织化学方法检测石蜡包埋人类前列腺癌组织，使用的抗体为PBK/TOPK Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PBK/TOPK Antibody preincubated with an irrelevant control peptide (left) or antigen specific peptide (right).免疫组织化学方法检测石蜡包埋人类乳腺癌组织，使用的抗体为PBK/TOPK Antibody，左图为共孵育无关对照多肽，右图为共孵育抗原特异性多肽。 | |
Immunohistochemical analysis of paraffin-embedded ME-180 cells, using PBK/TOPK Antibody (left), or no primary antibody (right).免疫组织化学方法检测石蜡包埋ME-180 细胞，左图为使用PBK/TOPK Antibody抗体，右图为不使用一抗的对照。 |
