| 货号 | 4068S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster/Zebrafish/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Pan-Cadherin Antibody detects endogenous levels of E-, P-, N-, R-, VE-, and K-Cadherins. |
| 使用方法 | WB(1:1000) IP (1:25) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 130 to 150 |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-cadherin Antibody.使用Pan-cadherin抗体对石蜡包埋的人前列腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-cadherin Antibody.使用Pan-cadherin抗体对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human bronchioalveolar cell carcinoma using Pan-cadherin Antibody, showing membrane localization.使用Pan-cadherin对石蜡包埋的人细支气管肺泡癌细胞进行免疫组化，显示细胞膜定位。 | |
Western blot analysis of extracts from A431, MCF-7, C2C12, HUVEC, BAEC, NIH/3T3, C6, H-4-II-E, Cos and PC3 cells, using Pan-Cadherin Antibody.使用Pan-cadherin抗体对A431, MCF-7, C2C12, HUVEC, BAEC, NIH/3T3, C6, H-4-II-E, Cos 和PC3 细胞提取物进行western blot分析。 |
