| 货号 | 4968S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Pan-Actin Antibody detects endogenous levels of total actin (all isoforms). The antibody also detects the 30 kDa actin fragment cleaved at glutamate 107. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from HeLa, L929, C6 and COS cells, using Pan-Actin Antibody.使用Pan-Actin Antibody，免疫印迹（Western Blot）分析HeLa、L929、C6、COS和COS细胞系Actin蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human leiomyoma, using Pan-Actin Antibody.使用Pan-Actin Antibody，免疫组化（Immunohistochemical）分析人源平滑肌瘤石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human heart, using Pan-Actin Antibody.使用Pan-Actin Antibody，免疫组化（Immunohistochemical）分析人源心脏组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using Pan-Actin Antibody.使用Pan-Actin Antibody，免疫组化（Immunohistochemical）分析人源骨骼肌石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon (smooth muscle), using Pan-Actin Antibody.使用Pan-Actin Antibody，免疫组化（Immunohistochemical）分析人源结肠（平滑肌）组织石蜡切片。 |
