| 货号 | 2602S |
| 反应种属 | Human/Mouse/Rat/Monkey/GuineaPig |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | PAK1 Antibody detects endogenous levels of total PAK1 protein. It does not cross-react with PAK2, PAK3 or other PAK family members. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10). |
| 存放说明 | -20C |
| 计算分子量 | 68 |
Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1 Antibody. 使用PAK1 Antibody，免疫印迹（Western Blot）分析SH-SY5Y、C6和NIH/3T3细胞中PAK1蛋白水平. | |
Immunoprecipitation of PAK1 from C6 and SH-SY5Y cells followed by Western blot analysis, using PAK1 Antibody. 使用PAK1 Antibody，免疫沉淀分析C6和SH-SY5Y细胞中PAK1蛋白，随后进行免疫印迹（Western Blot）实验。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using PAK1 Antibody. 使用PAK1 Antibody，免疫组化分析人源乳腺癌组织石蜡切片，结果显示细胞质定位。 | |
Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or PAK1 siRNA, using PAK1 Antibody #2602 and p42 MAP Kinase (Erk2) Antibody #9108. The PAK1 Antibody confirms silencing of PAK1 expression, and p42 MAP Kinase (Erk2) Antibody is used to control for loading and specificity of PAK1 siRNA. 使用PAK1 Antibody #2602和p42 MAP Kinase (Erk2) Antibody #9108，免疫印迹（Western Blot）分析HeLa细胞中PAK1和p42 MAP Kinase (Erk2)蛋白水平，细胞分为转染100 nM control siRNA #6201 (-)或PAK1 siRNA。PAK1 Antibody证实PAK1表达的沉默，而p42 MAP Kinase (Erk2) Antibody被用于内参照和PAK1 siRNA的特异性。 |
