| 货号 | 4992S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | PABP1 Antibody detects endogenous levels of total PABP1 and PABP3 proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | Poly(A)-binding protein 1 (PABP1) associates with the 3 poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5 cap structure (eIF4E), delivery of an RNA helicase to the 5 region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined. |
| 存放说明 | -20C |
| 计算分子量 | 71 |
Western blot analysis of extracts from HT-29 cells, using PABP1 Antibody. 使用PABP1 Antibody，免疫印迹（Western blot）分析HT-29细胞系提取物。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PABP1 Antibody. 使用PABP1 Antibody，免疫组化分析人类结肠癌组织的石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PABP1 Antibody. 使用PABP1 Antibody，免疫组化分析人类前列腺癌组织的石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using PABP1 Antibody. 使用PABP1 Antibody，免疫组化分析人类肾脏细胞癌组织的石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PABP1 Antibody. 使用PABP1 Antibody，免疫组化分析人类乳腺癌组织的石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using PABP1 Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 使用PABP1 Antibody，免疫组化分析肺癌组织的石蜡切片，分别孵育对照多肽 (左图)和抗原特异的多肽 (右图)。 |
