| 货号 | 4892S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40 kDa deltaNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms (3,4).p53肿瘤抑制蛋白在细胞DNA损伤和其他基因组异常的应答中起着重要的作用。 p53的激活可导致无论是细胞周期停滞和DNA修复或细胞凋亡（1）。除了p53，哺乳动物细胞中含有两个p53家族成员，p63和p73，其结构和功能都与p53蛋白类似（2）。虽然p63蛋白可诱导p53-应答基因与细胞凋亡，突变的p6 3很少引起肿瘤（2）。肺部和头颈部鳞状细胞癌中经常可以观察到p63基因的扩增（2,3）。 p63基因包含一个选择性的转录起始位点，该位点能够产生缺乏反式激活结构域的40 kDa的deltaNp63，且在羧基末端选择性剪接产生α，β和γ异构体（3,4）。 |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Western blot analysis of extracts from ME-180 cells, using p63α Antibody.Western blot 方法检测ME-180细胞，使用的抗体为 p63α Antibody。 | |
Confocal immunofluorescent analysis of A-431 cells (left) and MCF7 cells (right) using p63-α Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测A-431细胞(左图)和MCF7 cells (右图)，使用的抗体为p63-α Antibody，呈绿色。肌动蛋白纤维由DY-554鬼笔环肽标记，呈红色。 |
