| 货号 | 2557S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).p27 Kip1是周期蛋白依赖性激酶抑制剂Cip/Kip家族的一员。同其类似物一样，如p57 Kip2 和p21 Waf1/Cip1，其巩固G1限制点的能力源于CDK2/cyclin E和其它CDK/cyclin复合体的结合抑制。在静止期细胞和cAMP或其它细胞周期阴性调节剂处理的细胞中，p27的表达是上调的。白细胞介素2或者其它有丝分裂原能够诱导p27的下调；该过程涉及p27的磷酸化和泛素化-蛋白酶体途径介导的降解(1-4)。 |
| 存放说明 | -20C |
| 计算分子量 | 57 |
Western blot analysis of extracts from HeLa cells, untreated or treated with dexamethasone (50 nM, 16h) alone or with λ phosphatase, using p57 Kip2 Antibody #2557.Western blot 方法检测Hela细胞提取物，分为非处理组，地塞米松(50 nM) 单独处理16小时组和地塞米松及λ phosphatase共处理组，使用的抗体为 p57 Kip2 Antibody #2557。 | |
Western blot analysis, using a different p57 Kip2 mouse monoclonal antibody, of whole cell lysate from dexamethasone-treated (50 nM, 16h) HeLa cells (lane 1) and of p57 Kip2 immunoprecipitated from the same lysate using p57 Kip2 Antibody #2557 (lane 5). Lanes 2-4 represent controls for the immunoprecipitation experiment, as indicated.采用Western blot 方法检测地塞米松(50 nM) 单独处理16小时的Hela细胞（泳道1），使用的抗体为另一个不同的p57 Kip2鼠源单抗，以及使用p57 Kip2 Antibody #2557抗体免疫沉淀同一裂解液中的p57 Kip2蛋白（泳道5）。泳道2-4代表免疫沉淀实验中所需的对照。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or dexamethasone-treated (right), using p57 Kip2 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光检测Hela细胞，非处理组（左图）和地塞米松处理组（右图），使用的抗体为p57 Kip2 Antibody，呈绿色，肌动蛋白纤维由DY-554鬼笔环肽标记，呈红色。蓝色伪彩为DNA荧光染料，产品信息为DRAQ5® #4084。 |
