p42 MAP Kinase (Erk2) Antibody
货号：
9108S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 9108S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 42 |
参考图片Western blot analysis of extracts from various cell lines, using p42 MAP Kinase (Erk2) Antibody.Western blot方法检测细胞提取物：各种细胞系，使用的抗体是p42 MAP Kinase (Erk2) Antibody。 |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Pool p42 MAPK siRNA or single-duplex SignalSilence® p42 MAPK siRNA (+), using p42 MAP Kinase (Erk2) Antibody #9108 and α-Tubulin (11H10) Rabbit mAb #2125. The p42 MAP Kinase (Erk2) Antibody confirms silencing of p42 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p42 siRNA.Western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) 、SignalSilence® Pool p42 MAPK siRNA和single-duplex SignalSilence® p42 MAPK siRNA (+)的HeLa细胞。使用的抗体是p42 MAP Kinase (Erk2) Antibody #9108 和 α-Tubulin (11H10) Rabbit mAb #2125。p42 MAP Kinase (Erk2) Antibody证实了p42表达沉默，α-Tubulin (11H10) Rabbit mAb被当作负载及p42 siRNA的特异性控制来使用。 |
