| 货号 | 9218S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using p38α MAPK Antibody.western blot方法检测细胞提取物：HeLa, NIH/3T3, PC12 和COS细胞，使用的抗体是p38α MAPK Antibody。 | |
Western blot analysis of extracts from Xenopus oocytes overexpressing Myc-tagged p38 isoforms, using p38α MAPK Antibody (upper) and Myc-Tag Antibody (lower) to demonstrate the isoform specificity of p38α MAPK Antibody. (Provided by Dr. Eusebio Perdiguero and Dr. Angel Nebreda, European Molecular Biology Laboratory, Germany.)western blot方法检测细胞提取物：过量表达Myc标记p38异构体的卵母细胞 ，使用的抗体是 p38α MAPK Antibody (上图) 和Myc-Tag Antibody (下图)，以证实p38α MAPK Antibody的异构体特异性。(由 Dr. Eusebio Perdiguero and Dr. Angel Nebreda提供, European Molecular Biology Laboratory, Germany.) | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using p38α MAPK Antibody.免疫组织化学方法检测石蜡包埋的人乳腺癌组织，显示了细胞核、细胞质位点，使用的抗体为p38α MAPK Antibody。 |
