p190-B RhoGAP Antibody
货号：
2562S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 2562S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | p190-B RhoGAP Antibody detects endogneous levels of total RhoGAP protein. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Rho family of small GTPases, including Rho, Rac and Cdc42, are key regulators of diverse biological processes such as cytoskeletal organization, cell growth and differentiation, transcriptional regulation, and cell adhesion/motility (1,2). The activities of these proteins are controlled by GTP binding such that in the GTP-bound state they are active, and in the GDP-bound state they become inactive. Three classes of regulatory proteins control the activity of the GTPases by balancing the levels of GTP/GDP binding: the guanine nucleotide exchange factors (GEFs), which promote the active state by facilitating the exchange of GDP for GTP; the guanine nucleotide dissociation inhibitors (GDIs), which sequester the GDP-bound forms and may regulate their intracellular localization; and the GTPase activating proteins (GAPs), which increase GTP hydrolysis to promote the inactive state. The p190 RhoGAP family consists of two related proteins, p190-A and p190-B, which are both widely expressed and contain an amino-terminal GTPase domain and a carboxy-terminal RhoGAP catalytic domain. Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB (3). Phenotypes of these mice are similar to those of CREB knockouts, with reduced cell size, as well as defects in thymus and neural development (3). Cells deficient in p190-B also display defective adipogenesis, suggesting this pathway is critical for the "adipogenesis-myogenesis switch." (4). There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain (4-6). Levels of tyrosine phosphorylation are enhanced by Src overexpression (5,6). IGF-1 treatment downregulates Rho through phosphorylation and activation of p190-B RhoGAP, thereby enhancing IGF signaling implicated in adipogenesis (4). |
| 存放说明 | -20C |
| 计算分子量 | 190 |
参考图片Western blot anlaysis of extracts from MCF-7 (human), C2C12 (mouse), and BAEC (bovine) cells, using p190-B RhoGAP Antibody. 使用p190-B RhoGAP Antibody，免疫印迹（Western Blot）分析MCF-7 (human)、C2C12 (mouse)和BAEC (bovine)细胞中p190-B RhoGAP蛋白水平。 |
