| 货号 | 14127S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 42, 45 |
Western blot analysis of extracts from various cell lines using OTULIN Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human OTULIN protein (hOTULIN-Myc/DDK; +) or Myc/DDK-tagged full-length human OTUB1 protein (hOTUB1-Myc/DDK; +), using OTULIN Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower). | |
Immunoprecipitation of OTULIN from MCF7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or OTULIN Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using OTULIN Antibody. | |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence®OTULIN siRNA I #14132 (+), using OTULIN Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The OTULIN Antibody confirms silencing of OTULIN expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control. |
