| 货号 | 3888S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).核有丝分裂器蛋白(NuMA)是一种参与有丝分裂纺锤体形成和维持的卷曲蛋白。NuMA在分裂间期的染色质组织过程起其重要作用，从而影响乳腺细胞分化(1,2)。凋亡过程中，NuMA的羧基末端清除可以放大细胞死亡信号通路。NuMA含有多个磷酸化位点，ATM/ATR依赖方式发生的丝氨酸395位磷酸化能够响应DNA损伤(3,4)。 |
| 存放说明 | -20C |
| 计算分子量 | 238 |
Western blot analysis of extracts from various cell types using NuMA Antibody.western blot检测不同细胞提取物，使用的抗体为NuMA Antibody. | |
Confocal immunofluorescent analysis of HeLa cells using NuMA Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光方法检测HeLa细胞，检测抗体为NuMA Antibody ，呈绿色。肌动蛋白纤维由DY-554 鬼笔环肽标记，呈红色。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 |
