| 货号 | 14810S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Both the NEDD8 ultimate buster 1 (NUB1) and the related NUB1L isoform are interferon-inducible adaptor proteins that negatively regulate ubiquitin-like protein NEDD8 (1,2). NUB1 protein contains an amino terminal ubiquitin-like (UBL) domain and multiple carboxy terminal ubiquitin-associated (UBA) domains. The NUB1L isoform is generated by alternative splicing and contains an extra UBA domain relative to NUB1 (2). Research studies indicate that NUB1 and NUB1L non-covalently bind NEDD8 and facilitate delivery of both NEDD8 monomers and NEDD8 conjugates to the proteasome for degradation (2-5). In addition, NUB1L binds and enhances the proteasomal degradation of the FAT10 ubiquitin-like protein (6). Additional research shows that NUB1 negatively regulates cell proliferation, likely due to inhibition of NEDD8 conjugation to SCF ubiquitin ligases, which leads to inhibition of p27 and cyclin E ubiquitination (3,7). NUB1 has been identified as a putative therapeutic target in Huntingtons disease as NUB1 promotes a decrease in levels of mutant HTT protein (8). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from various cell lines using NUB1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, U-2 OS cells express low levels of endogenous NUB1 protein (3). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NUB1L protein (hNUB1L-Myc/DDK; +), using NUB1 Antibody. | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72hr; +), using NUB1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). |
