| 货号 | 2889S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Notch3 Antibody detects endogenous levels of total Notch3 protein. It recognizes the full-length (270 kDa) and the extracellular truncated fragment containing a short extracellular region, the transmembrane domain and the intracellular region (90 kDa). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 90, 270 |
Western blot analysis of extracts from A2780 and HBP-ALL cells using Notch3 Antibody. 使用Notch3抗体对A2780和HBP-ALl细胞提取物进行western blot分析。 |
