| 货号 | 9405S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | N-Myc Antibody detects endogenous levels human N-Myc and transfected levels of mouse N-Myc. It does not cross-react with other Myc family members. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
Western blot analysis of extracts from the IMR-32 neuroblastoma cell line, using N-Myc Antibody. 使用 N-Myc 抗体对 IMR-32 成神经细胞瘤的细胞系的提取物免疫印迹分析 | |
Western blot analysis of extracts from HeLa cells either mock transfected or transfected with murine N-Myc, using N-Myc Antibody. 采用 N-Myc Antibody抗体对模拟转染或用鼠类N-Myc转染的Hela细胞进行Western Blot分析。 |
