| 货号 | 3345S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | NME1/NDKA (D98) Antibody detects endogenous levels of total NME1/NDKA protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including protein-protein interactions (4-6), regulation of GTP-binding protein function (7-9), DNA-associated activities (10,11), and histidine-dependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied (14). NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKα1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKα1. This regulates AMPKα1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NME1/NDKA (D98) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组织化学检测石蜡包埋的人肺癌组织，所用抗体为NME1/NDKA (D98) Antibody。左为对照肽，右为抗原特异性肽。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using NME1/NDKA (D98) Antibody. 免疫组织化学检测石蜡包埋的人淋巴瘤组织，所用抗体为NME1/NDKA (D98) Antibody。 | |
Western blot analysis of extracts from various cell lines using NME1/NDKA (D98) Antibody. Wetern blot检测多种细胞提取物，所用抗体为NME1/NDKA (D98) Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using NME1/NDKA (D98) Antibody. 免疫组织化学检测石蜡包埋的人乳腺癌组织，所用抗体为NME1/NDKA (D98) Antibody。 |
