NIPA Antibody
货号：
4497S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 4497S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | NIPA (nuclear interaction partner of ALK) is an F-box-containing protein that is an essential component of the SCF-type E3 ligase (SCFNIPA) complex, a complex that controls the completion of S-phase and mitotic entry (1). This control is mediated by the ubiquitination and subsequent degradation of cell cycle regulatory proteins, whose oscillation of protein levels is required for proper cell cycle progression (2).
Expression levels of NIPA are low in G0/G1 phases and upregulated in S and G2/M phases. The SCFNIPA complex targets nuclear cyclin B1 for ubiquitination in interphase, whereas phosphorylation of NIPA in late G2 phase and mitosis inactivates the complex to allow for accumulation of cyclin B1 (3). NIPA may have an anti-apoptotic role in NPM-ALK-mediated signaling events (4).NIPA是一种F-box蛋白，在SCF型E3连接酶(SCFNIPA)复合体的组成中必不可少，该复合体能够控制S期的完成和有丝分裂的进入(1)。这种控制是通过泛素化和随后的细胞周期调节蛋白降解来介导的，合适的细胞周期进程需要蛋白水平的波动(2)。NIPA的表达水平在G0/G1期较低，而在S期和G2/M期表达上调。SCFNIPA复合体靶向核周期蛋白B1，促进分裂间期的泛素化，而G2末期和有丝分裂期的NIPA磷酸化能够使复合体失活从而促进周期蛋白B1的累积(3)。NIPA也可以在NPM-ALK介导的信号事件中发挥抗凋亡的作用(4)。 |
| 存放说明 | -20C |
| 计算分子量 | 60-65 |
参考图片Western blot analysis of extracts from HeLa cells, either untreated (asynchronous) or treated with nocodazole or hydroxyurea, using NIPA Antibody.western blot方法检测HeLa细胞提取物：未处理组（非同步）、nocodazole处理组、羟基脲处理组，使用的抗体为NIPA Antibody。 |
