| 货号 | 3394S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | NHERF1 (A310) Antibody detects endogenous levels of total NHERF1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Na+/H+ exchanger regulatory factor (NHERF1 or EBP-50) is one of several related PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains and with the ERM family of proteins including merlin via its carboxy-terminus (2,3). NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells (2,3). Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1 and PDGFR. Thereby NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of the PI3K/Akt signaling in normal cells, and this mechanism may be disrupted in cancer (4). NHRF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in Cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting and trafficking (6). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Western blot analysis of extracts from MCF-7 and T-47D cells using NHERF1 (A310) Antibody. Western blot分析MCF-7和T-47D细胞提取物，使用的抗体是NHERF1 (A310) Antibody 兔多抗。 | |
Projected confocal z-stack of MCF-7 cells showing staining at the apical membrane when labeled with NHERF1 (A310) Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). MCF-7细胞的共聚焦z-stack显示，使用 NHERF1 (A310) Antibody 兔多抗（绿色）标记时，在顶端胞膜有染色。蓝色伪彩=DRAQ5 ® #4084(荧光DNA染料)。 |
