| 货号 | 4389S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | NFAT1 Antibody detects endogenous levels of total NFAT1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response (1,2). NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells (3). In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus (2). Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1 (3,4). Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm (5). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Confocal immunofluorescent analysis of Jurkat cells (left) and HeLa cells (right) using NFAT1 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析Jurkat细胞 (左图)和HeLa细胞(右图) ，所用抗体为NFAT1 Antibody(绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from various cell lines using NFAT1 Antibody.Western免疫印迹。用NFAT1 Antibody研究各种细胞系的细胞提取液。 |
