| 货号 | 11867S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | NBC1/SLC4A4 Antibody recognizes endogenous levels of total NBC1 protein. This antibody also cross-reacts with 42 and 62 kDa proteins of unknown origin in some cell lines. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | NBC1/SLC4A4 is an eletrogenic sodium bicarbonate cotransporter. It mediates the coupled movement of Na+ and HCO3- ions across the basolateral membrane of cells. NBC1 regulates secretion and absorption of bicarbonate and modulates intracellular pH (1-3). There are five isoforms of NBC1 produced by alternative splicing including the most studied isoforms, 1 and 2, which have different N termini. The N terminus of isoform 1 contains multiple consensus phosphorylation sites for various kinases, such as PKA, PKC, and CK II, and may play a regulatory role in the biological function of NBC1 (4). Interaction with carbonic anhydrase II, IV, and IX regulates the transporter acitivity of NBC1 (5-7). NBC1 is expressed in various tissues with the exception of isoform 2, which is mainly expressed in kidney proximal tubules (1). Isoform 1 is expressed in the pancreas and corneal endothelium, and at low levels in other tissues including heart, skeletal muscle, liver, and prostate (4). Research studies have shown that mutations in the NBC1 gene are linked to proximal renal tubular acidosis with ocular abnormalities (also known as renal tubular acidosis II). |
| 存放说明 | -20C |
| 计算分子量 | 135-145 |
Western blot analysis of extracts from LNCaP and LN18 cells using NBC1/SLC4A4 Antibody. Western blot 检测 LNCaP 和 LN18细胞系提取物，使用抗体为NBC1/SLC4A4 Antibody。 | |
Immunoprecipitation of NBC1/SLC4A4 from LNCaP cell extracts, using Normal Rabbit IgG #2729 (lane 2) or NBC1/SLC4A4 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NBC1/SLC4A4 Antibody. 免疫沉淀方法检测LNCaP细胞提取物NBC1/SLC4A4，使用抗体为Normal Rabbit IgG #2729 (泳道2)或NBC1/SLC4A4 Antibody (泳道3)。泳道1为10%对照。使用NBC1/SLC4A4 Antibody进行Western blot分析。 |
