货号 | 2262S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP |
目标/特异性 | Naked1 antibody detects endogenous levels of total Naked1 proteins. |
使用方法 | WB(1:1000) IP (1:50) |
供应商 | CST |
背景 | Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6). |
存放说明 | -20C |
计算分子量 | 59, 61 |
Western blot analysis of total cell extracts of SW620 and HepG2 cells, using Naked1 Antibody. 使用Naked1抗体对SW620和HepG2细胞全细胞提取物进行western blot分析。 |