| 货号 | 3402S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | Myosin Va Antibody detects endogenous levels of total myosin Va heavy chain. Based on sequence homology, the antibody is expected to detect all known myosin Va splice variants. |
| 使用方法 | WB(1:1000) IP (1:100) IF-F (1:50) |
| 供应商 | CST |
| 背景 | Myosin Va is a molecular motor protein involved in the transport of organelles, vesicles and other cellular cargo along actin filaments (reviewed in 1). The molecule consists of two identical heavy chains, which dimerize via helical domains in a coiled coil structure. The amino-terminal motor domains of the heavy chains contain both the ATPase and the actin-binding activities of myosin Va. The globular tail domains act in a regulatory capacity, binding the myosin Va cargo (2) or inhibiting motor activity by binding the head domains and preventing ATP consumption (3). Mutation of the murine dilute gene, which encodes myosin Va, causes defects in coat pigmentation as well as severe neurological defects (4). In melanocytes, the coiled coil structure of myosin Va is important in regulating the trafficking of melanosomes in conjunction with melanophilin and Rab27a (5). Myosin Va regulates trafficking and exocytosis of secretory granules in neuroendocrine cells (reviewed in 6) as well as RNA transport and distribution (7). |
| 存放说明 | -20C |
| 计算分子量 | 207 |
Western blot analysis of extracts from SK-MEL-28 and C2C12 cells using Myosin Va Antibody. 使用Myosin Va Antibody，免疫印迹（Western Blot）分析SK-MEL-28和C2C12细胞中Myosin Va蛋白水平。 | |
Confocal immunofluorescent analysis of the hippocampus (left) and pons region (right) of adult rat brain using Myosin Va Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Myosin Va Antibody (绿色)标记，共聚焦免疫荧光分析成年大鼠大脑hippocampus (左图)和pons region (右图)。蓝色伪彩= DRAQ5™（DNA荧光染料）。 |
