| 货号 | 3404S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Myosin IIb Antibody detects endogenous levels of total myosin IIb protein. The antibody does not cross-react with the nonmuscle heavy chains of myosin IIa or IIc. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
Western blot analysis of extracts from various cell types using Myosin IIb Antibody. 使用Myosin IIb Antibody，免疫印迹（Western Blot）分析不同细胞中Myosin IIb蛋白水平。 | |
Western blot analysis of extracts from COS cells, untransfected or transfected with nonmuscle EGFP-myosin IIa, IIb or IIc, using Myosin IIb Antibody. 使用Myosin IIb Antibody，免疫印迹（Western Blot）分析COS细胞中Myosin IIa蛋白水平。细胞分为mock transfected、转染EGFP-myosin IIa、IIb或IIc。 | |
Confocal immunofluorescent analysis of Cos cells using Myosin IIb Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Myosin IIb Antibody (绿色)标记进行共聚焦免疫荧光分析Cos细胞。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5™（DNA荧光染料）。 |
