货号 | 2071S |
反应种属 | Mouse |
来源宿主 | Rabbit |
应用 | W/IP/IHC-P/IF-F |
目标/特异性 | Mili Antibody detects endogenous levels of total Mili protein. |
使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) IF-F (1:200) |
供应商 | CST |
背景 | The Drosophila piwi gene was identified as being required for the self-renewal of germ-line stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and human (1). Miwi and Mili proteins are both mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mouse (3-5). |
存放说明 | -20C |
计算分子量 | 110 |
Confocal immunofluorescent analysis of mouse testis using Mili Antibody (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Mili Antibody(绿色)和Pan-Keratin (C11) Mouse mAb 鼠单抗#4545(红色),共聚焦免疫荧光观察Mili蛋白在小鼠睾丸组织中定位。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料). | |
Western blot analysis of extracts from various mouse tissues using Mili Antibody (upper panel) or β-Actin Antibody (#4967) (lower panel). 使用Mili Antibod (上图)和β-Actin Antibody #4967 (下图),免疫印迹(Western blot)分析小鼠不同组织的提取物。 | |
Immunohistochemical analysis of paraffin-embedded mouse testis using Mili Antibody. |