| 货号 | 2823S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | MEP50 Antibody detects endogenous levels of total MEP50 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | MEP50 (methylosome protein 50) is a component of the methylosome, a protein arginine methyltransferase complex that modifies specific arginine residues found in arginine- and glycine-rich regions of some spliceosomal Sm proteins. MEP50 is important for methylosome activity and may regulate the transfer of Sm proteins to the SMN (survival of motor neurons) complex, an early step in the assembly of U snRNPs. Both the methylosome and the SMN complex are essential for the assembly of spliceosomal snRNPs (1). MEP50 is a WD repeat protein that may provide an interface for multiple protein interactions between methylosome proteins. (1). It binds to JBP1, an arginine protein methyltransferase component of the methylosome. MEP50 has been shown to interact with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing (2), and with SUZ12, a polycomb group protein involved in transcriptional repression (3). JBP1 and MEP50 have also been reported to interact with the methyl-CpG binding protein complex MBD2/NuRD (4). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
Western blot analysis of extracts from U2OS and HeLa cells using MEP50 Antibody. 使用MEP50 Antibody，免疫印迹(Western blot)分析U2OS和HeLa细胞中MEP50的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using MEP50 Antibody (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用MEP50 Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY554 phalloidin标记微丝蛋白(红色)。蓝色= DRAQ5® #4084 (DNA荧光染料)。 |
