| 货号 | 4983S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/IF-IC |
| 目标/特异性 | MAVS Antibody (Rodent Specific) detects endogenous levels of total MAVS/VISA protein. The bands detected at 52 and 75 kDa correlate with those described by Seth et al. (2005). |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 75, 52 |
Western blot analysis of extracts from A20 and CTLL-2 cell lines using MAVS Antibody (Rodent Specific). | |
Confocal immunofluorescent analysis of an A20 cell using MAVS Antibody (Rodent Specific) (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).共聚焦免疫荧光分析A20细胞，所用抗体为MAVS Antibody (Rodent Specific)(绿色)，图中所示为线粒体的共定位，用 MitoTracker® Red CMXRos (红色)标记。Blue pseudocolor =DRAQ5™(DNA荧光染料)。 |
